In vivo imaging of the human cornea with high-speed and high-resolution Fourier-domain full-field optical coherence tomography

Biomedical Optics Express  2020 | Vol. 11, No.5 | 2849-2865 

Egidijus Auksorius, Dawid Borycki, Patrycjusz Stremplewski, Kamil Liżewski, Sławomir Tomczewski, Paulina Niedźwiedziuk, Bartosz L. Sikorski and Maciej Wojtkowski


Corneal evaluation in ophthalmology necessitates cellular-resolution and fast imaging techniques that allow for accurate diagnoses. Currently, the fastest volumetric imaging technique is Fourier-domain full-field optical coherence tomography (FD-FF-OCT), which uses a fast camera and a rapidly tunable laser source. Here, we demonstrate high-resolution, high-speed, non-contact corneal volumetric imaging in vivo with FD-FF-OCT that can acquire a single 3D volume with a voxel rate of 7.8 GHz. The spatial coherence of the laser source was suppressed to prevent it from focusing on a spot on the retina, and therefore, exceeding the maximum permissible exposure (MPE). The inherently volumetric nature of FD-FF-OCT data enabled flattening of curved corneal layers. The acquired FD-FF-OCT images revealed corneal cellular structures, such as epithelium, stroma and endothelium, as well as subbasal and mid-stromal nerves.

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This project has received funding from the European Union’s
Horizon 2020 research and innovation programme
under grant agreement No 666295.