Study & working visits
The purpose of the visit was twofold: for the new group members to learn the experimental setup and procedure of two-photon vision threshold measurement and to verify applicability of an existing product of Fluence, manufacturer of femtosecond fibre lasers, for this technique. An extra target was to explore requirements of the method and setup to start the process of adapting the Fluence laser when it comes to integration into the measurement device.
The purpose of this business trip was to perform collaborative work on the interferometric near-infrared spectroscopy (iNIRS). More specifically, experiments to quantify optical properties in dynamic media were performed. Then, the data was processed numerically using the new method of correlation gating to separate ballistic and scattered light transmitted through thick samples.
Together with other members of the group Jan Guzowski visited Prof. Roland Zengerle Lab at University of Freiburg, Germany. The agenda of the meeting included presentation by Prof. Zengerle as well as by other two group leaders in his lab, namely Dr. Peter Koltay and Dr. Felix von Stetten. The purpose of the visit was to establish long-term collaboration with prof. Garstecki lab, in particular associated with co-advising by prof. Zengerle of two PhD students, Yu-Ting Kao and Yu-Kai Lai.
Yuriy Stepanenko visited the University of Nevada, Reno. The purpose of this study visit was to continue the collaboration began in 2007 with the group of Laser-plasma interaction at Nevada Terawatt Facility, University of Nevada, Reno. The group uses advanced laser sources for plasma generation, particle acceleration and diagnostics of Z-pinch.
This visit was scheduled in cooperation with one of the best group in the field of ultrafast Optical Coherence Tomography (OCT) – prof. Huber group from Lubeck. The purpose of the visit was to obtain results from ultrafast (up to 100 vol./s) OCT on rabbit and mice hearts. In order to do that, we have made a cooperation with Max Planck for Dynamics and Self-Organization from Goettingen, which gave us a support from biological point of view (also provided with well-prepared hearts).
The goal of the visit was to carry first in the world investigation of full heart with use of OCT system. For that Michał Hamkało had to get newly extracted hearts of mice from an authorized laboratory in Goettingen and took it to Luebeck (to next cooperator) with help of dr Jan Christoph. In the Institute for Biomedical Optics in Luebeck he was working with the OCT system (one of the fastest in the world) in order to specify parameters of heart imaging.
The goal of the visit was to perform experiments on human cells using novel microscopic technique: differential dynamic microscopy (DDM). The plan of the experiments contained quantification of movement of subcellular components in native HeLa cells and observation of changes of mobility during programmed cell death (apoptosis).
The purpose of the travel was to assess the perspectives for future collaboration with the Soft Living Matter Group run by prof. Clifford Brangwynne at the Princeton University. The group conducts cutting-edge research in the field of physicochemistry of soft matter in living systems. The group possesses a wide background and know-how concerning biophysical studies on model biological systems at all complexity levels (single cells, tissues and organisms). It is applied there, i.e., to studies of phase transitions in living systems and intrinsically disordered proteins, which play a key role in the pathogenesis of neurodegenerative diseases.
Professor Maciej Wojtkowski (the ERA Chair holder, the Head of the Department of Physical Chemistry of Biological Systems) accompanied by the:Director of the Institute of Physical Chemistry PAS - professor Marcin Opallo, President of the Scientific Council of IPC - professor Aleksander Jablonski Project Coordinator - professor Robert Holyst, and former postdoc at Professor Holyst Group – Associate Professor Hou Sen, linking person with Nankai University,
visited Nankai University in Tianjin, China (the 6 – 8th March, 2017).
Two-photon excitation fluorescence (TPEF) imaging of the back of the eye allows visualization of subcellular structures in the living animal eye. This method is helpful for investigating mechanisms of retinal diseases and development of ophthalmic therapies. Endogenous fluorophores, necessary for replenishing visual chromophore, and thus sustaining vision have absorption maxima in the range from 320 – 400 nm. However, anterior optics of the animal eye poorly transmit light at those wavelengths. Two-photon excitation fluorescence imaging employing 75 fs laser pulses overcomes this barrier and visualizes subcellular organelles in the living animal eye.