Study & working visits
Two-photon excitation fluorescence (TPEF) imaging of the back of the eye allows visualization of subcellular structures in the living animal eye. This method is helpful for investigating mechanisms of retinal diseases and development of ophthalmic therapies. Endogenous fluorophores, necessary for replenishing visual chromophore, and thus sustaining vision have absorption maxima in the range from 320 – 400 nm. However, anterior optics of the animal eye poorly transmit light at those wavelengths. Two-photon excitation fluorescence imaging employing 75 fs laser pulses overcomes this barrier and visualizes subcellular organelles in the living animal eye.
Working visit to Optical Biomedical Imaging Group Institute of Physics, Nicolaus Co-pernicus University.
The visit to Optical Biomedical Imaging Group Institute of Physics, Nicolaus Copernicus University was to have the deep analysis of the in-vivo imaging of the global mouse brain ischemia (GI) using Bessel beam optical coherence microscopy. This method allows to monitor changes in brain structure with extra control of blood flow during the process of artery occlusion. The results showed the capability and sensitivity of OCM system with Bessel beam to analyze brain plasticity after severe injury within a period of 8 days.