Longitudinal in-vivo OCM imaging of glioblastoma development in the mouse brain
Biomedical Optics Express 2020 | Vol. 11, Issue 9 | pp. 5003-5016
Dolezyczek H., Rapolu M., Niedzwiedziuk P., Karnowski K., Borycki D., Dzwonek J., Wilczynski G., Malinowska M., Wojtkowski M.
We present in-vivo imaging of the mouse brain using custom made Gaussian beam optical coherence microscopy (OCM) with 800nm wavelength. We applied new instrumentation to longitudinal imaging of the glioblastoma (GBM) tumor microvasculature in the mouse brain. We have introduced new morphometric biomarkers that enable quantitative analysis of the development of GBM. We confirmed quantitatively an intensive angiogenesis in the tumor area between 3 and 14 days after GBM cells injection confirmed by considerably increased of morphometric parameters. Moreover, the OCM setup revealed heterogeneity and abnormality of newly formed vessels.