Lab visit to the Institute for Laser Technology in Medicine and Measurement Technique
Yu-Ting Kao, Ph.D. student, visited the Institute for Laser Technology in Medicine and Measurement Technique in Germany. The aim of the visit was to obtain some useful information that will become a reference in her Ph.D. project. The first object was to find out the limit of detection (LOD) of the Light-up Player by using the well-known fluorophores and recondition the software connected to the Light-up Player to be more user-friendly. Light-up Player system has been developed by the Institute for Laser Technology in Medicine and Measurement Technique (ILM) for the droplet detection of recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) assays. However, it has not been examined in fluorescence in situ hybridization (FISH) assay which is part of Yu-Ting Kano research.
In addition, it is necessary to develop a novel and fast method for cell permeabilization in the droplet-FISH assay. In order to let developed assay be more competitive in the market, sensitivity, selectivity, and time-to-results are crucial. If a single laser pulse can efficiently permeabilize cells and at the same time transferring interesting markers to the targeted cell, this could shorten the time frame of the overall assay.
The second object was to support CellPulse which is a project using a single laser pulse to transfer molecules/plasmids into immunocytes (e.g. THP-1 cells) for biomedical applications. This also can be applied to the microfluidic platforms for further purposes.
15 Sep.-14 Dec. 2019
Institute for Laser Technology in Medicine and Measurement Technique (ILM), Ulm, Germany